√在线天堂中文最新版网,97se亚洲综合色区,国产成人av免费网址,国产成人av在线影院无毒,成人做爰100部片

×

multiple cloning sites中文什么意思

發(fā)音:   用"multiple cloning sites"造句
  • 多克隆位點(diǎn)
  • multiple:    adj. 1.多重的；復(fù)合的,復(fù)式的,多數(shù)的,多樣的。 ...
  • clone:    n. 1.【生物學(xué)】純種細(xì)胞，無性系。 2.復(fù)制品；(幾 ...
  • site:    n. 1.地點(diǎn)；位置；地基。 2.場(chǎng)所，現(xiàn)場(chǎng)。 3.遺址 ...
  • polylinker cloning sites:    多接頭克隆位點(diǎn)
  • multiple cloning site:    多克隆位點(diǎn)
下載手機(jī)詞典可隨時(shí)隨地查詞查翻譯

例句與用法

    更多例句：  下一頁
  1. According to csfv ' s e2 gene sequence published in genbank and the sequence of p . pastoris expression vector ppic9 ' s multiple cloning site ( mcs ) , a pair of primers were designed with oligo and primers . o softwares
    將該基因片段先克隆到pmd18 - t載體上，并進(jìn)行了酶切、 pcr鑒定和測(cè)序分析，陽性重組質(zhì)粒命名為t - e2 。
  2. Construction of two vectors containing different plasmid original replicon this work constructed four resolution shuttle vectors , pbmb1205 , pbmb1205r , pbmb1206 and pbmb1206r . there are multiple clone sites between two copies of res sites
    解離載體的構(gòu)建和性能利用來源于不同質(zhì)粒上的質(zhì)粒復(fù)制起始區(qū)ori44和ori1030構(gòu)建了4個(gè)解離載體。
  3. The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak . 8 . bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
    將克隆到的hbmp基因通過適當(dāng)?shù)拿盖胁迦氲睫D(zhuǎn)移載體質(zhì)粒pbac - pak8的多克隆位點(diǎn)中，獲得重組轉(zhuǎn)移載體質(zhì)粒pbacpak - hbmp 。
  4. The interest gene was inserted in the - tha l . tho 1 multiple cloning sites of donor plasimd pfastbachtb of baculovirus expression system . after analysis by restriction endonuclease and pcr , the recombinant donor plasmid gpl - fast was transforn1ed to the competent celi dhi0bac which contalns the bacmid and the helper plasmid , the recombinant bacmid gpl - bac was acquired which would express the vpl of gpv strain h l
    同時(shí)將該目的基因插入到桿狀病毒表達(dá)系統(tǒng)的供體質(zhì)粒pf _ ( ast ) b _ ( ac ) htb的xba 、 xho多克隆位點(diǎn)間，經(jīng)酶切、 pcr鑒定后，將重組的供體質(zhì)粒gp1 - fast轉(zhuǎn)化到含有桿狀病毒和輔助質(zhì)粒的dh10b _ ( ac )感受態(tài)細(xì)胞中，獲得了表達(dá)gpvh1株vp1的重組桿狀病毒gp1 - bac 。
  5. A bt - e . coli shuttle vector pht315 was deleted its replication region of bt , then constructed a novel vector named pht315 - 1 which composed a multiple cloning site , erythromycin and ampicillin - resistance marker and could only replicated in e . coli . used pht315 - 1 , a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324 . sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501 , 333 , 183aas . orfl had 98 % identities with replicating related protein ori43 of bt strain hd263 . the others were no homology to any published bt replicating related protein . after continuous cultured for 70h at 30 c without antibiotic selecting press . the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 % . and growth curve also showed that the novel replicon was stable and could replicate normally
    進(jìn)一步序列分析表明該復(fù)制區(qū)至少有3個(gè)較大的orf ，分別編碼501 ， 333 ， 183個(gè)氨基酸。其中orf1蛋白序列與hd263復(fù)制蛋白o(hù)ri43的同源性為98 ，而另外兩個(gè)orf和genbank己公布的bt復(fù)制相關(guān)蛋白無同源性。 30連續(xù)培養(yǎng)72h ，復(fù)制區(qū)質(zhì)粒在bt無晶體突變株hd73cry ~ -中穩(wěn)定性達(dá)98以上， 30h生長(zhǎng)曲線也表明該復(fù)制區(qū)能夠在bt中穩(wěn)定復(fù)制和遺傳，對(duì)受體菌株無明顯不良影響。

相關(guān)詞匯

相鄰詞匯

  1. multiple cleaning 什么意思
  2. multiple clear cell acanthoma 什么意思
  3. multiple cleavage 什么意思
  4. multiple clone site 什么意思
  5. multiple cloning site 什么意思
  6. multiple closed dislocations of arm 什么意思
  7. multiple closed dislocations of back 什么意思
  8. multiple closed dislocations of hand 什么意思
  9. multiple closed fractures of hand bones 什么意思
  10. multiple cloth 什么意思
桌面版繁體版English

相關(guān)閱讀

Copyright ? 2025 WordTech Co.